![]() Trophectoderm potency is retained exclusively in human naïve cells. Naïve human pluripotent stem cells respond to Wnt, Nodal and LIF signalling to produce expandable naïve extra-embryonic endoderm. Derivation of trophoblast stem cells from naïve human pluripotent stem cells. Generation of blastocyst-like structures from mouse embryonic and adult cell cultures. Self-organization of mouse stem cells into an extended potential blastoid. Induced 2C expression and implantation-competent blastocyst-like cysts from primed pluripotent stem cells. Blastocyst-like structures generated solely from stem cells. An in vitro model of early anteroposterior organization during human development. Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells. Controlled modelling of human epiblast and amnion development using stem cells. A pluripotent stem cell-based model for post-implantation human amniotic sac development. A method to recapitulate early embryonic spatial patterning in human embryonic stem cells. Warmflash, A., Sorre, B., Etoc, F., Siggia, E. A 3D model of a human epiblast reveals BMP4-driven symmetry breaking. Self-organization of a human organizer by combined Wnt and Nodal signalling. ![]() ![]() Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Induced pluripotent stem cell lines derived from human somatic cells. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Reprogramming of human somatic cells to pluripotency with defined factors. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Embryonic stem cell lines derived from human blastocysts. Human blastoids provide a readily accessible, scalable, versatile and perturbable alternative to blastocysts for studying early human development, understanding early pregnancy loss and gaining insights into early developmental defects. Using chemical perturbations, we show that specific isozymes of protein kinase C have a critical function in the formation of the blastoid cavity. Human blastoids are amenable to embryonic and extra-embryonic stem cell derivation and can further develop into peri-implantation embryo-like structures in vitro. Single-cell RNA-sequencing analyses also reveal the transcriptomic similarity of blastoids to blastocysts. These structures-which we term ‘human blastoids’-resemble human blastocysts in terms of their morphology, size, cell number, and composition and allocation of different cell lineages. Starting from naive human pluripotent stem cells, here we developed an effective three-dimensional culture strategy with successive lineage differentiation and self-organization to generate blastocyst-like structures in vitro. However, models of the pre-implantation human blastocyst are lacking. Recent advances in partial embryo models derived from human pluripotent stem cells have enabled human development to be examined at early post-implantation stages 8, 9, 10, 11, 12, 13, 14. Human pluripotent stem cells provide an alternative means to study human development in a dish 1, 2, 3, 4, 5, 6, 7. Limited access to embryos has hampered the study of human embryogenesis and disorders that occur during early pregnancy.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |